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S0751 Sigma-Aldrich

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

Synonym: Monosodium dihydrogen orthophosphate, Monosodium phosphate, Sodium dihydrogen phosphate

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, HIS Select Supporting Products and Reagents, HIS-Select,
grade   ReagentPlus®
InChI Key   AJPJDKMHJJGVTQ-UHFFFAOYSA-M
assay   ≥99.0%
form   powder
pH   4.0-4.5 (25 °C, 50 g/L in water)
pKa (25 °C)   (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

Description

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

3 kg in poly drum

Application

Sodium phosphate monobasic has been used:
• as a reagent for virus transfection and fiber implantation in mouse
• in phosphate buffer for immunocytochemistry and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)
• as a buffer for cycling assay
• in automated DNA extraction method
• as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats

General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD007

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
WA1900000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of ...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

UHPLC Analysis NIST mAb Variants on Proteomix SCX-NP5: Comparing Salt and pH Gradients

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis NIST mAb Variants on Proteomix SCX-NP5: Comparing Salt and pH Gradients
Keywords: Chromatography, Ion Exchange, Separation

UHPLC Analysis of NIST mAb Charge Variants: Effect of Particle and Ligand Morphology

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of NIST mAb Charge Variants: Effect of Particle and Ligand Morphology
Keywords: Chromatography, Gene expression, Ion Exchange

UHPLC Analysis of NIST mAb on Zenix SEC-300

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of NIST mAb on Zenix SEC-300
Keywords: Chromatography, Size-exclusion chromatography

Protocols

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Trypsin Inhibitor

3.2 Unit Definition – One Trypsin BAEE unit will produce a ΔA253nm of 0.001 per minute with BAEE as substrate at pH 7.6 at 25ºC in a reaction volume of 3.2 ml.

Enzymatic Assay of Trypsinogen

This scope of this procedure applies to products that have a specification for the enzymatic activity of trypsinogen.
Keywords: Enzyme activity, Extinction coefficient

Enzymatic Assay of Urease from Jack Beans

The scope of this procedure is for all products that have a specification for the enzymatic determination of Urease, from Jack Beans. This assay is not to be used to determine the activity of Urease,...
Keywords: Indicators, Sonication, Titrations

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for determining Trypsin activity using Nα-Benzoyl-L-arginine ethyl ester (BAEE) as the substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light pa...

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Sodium phosphate monobasic (s1751) Discussion and Reviews

If you would like to leave a review, question, application info, or other comment, follow this Link.

Peer-Reviewed Papers
15

References

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