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T4375 Sigma-Aldrich

Tetrazolium Blue Chloride

used in colorimetric determination of reducing compounds

Synonym: 3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

  • CAS Number 1871-22-3

  • Empirical Formula (Hill Notation) C40H32Cl2N8O2

  • Molecular Weight 727.64

  •  Beilstein/REAXYS Number 3894077

  •  EC Number 217-488-8

  •  MDL number MFCD00040933

  •  PubChem Substance ID 24900207

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Properties

Related Categories Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes, T-U-V More...
Quality Level   200
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   255 °C (dec.) (lit.)
solubility   methanol: 50 mg/mL, clear
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8
InChI   1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2
InChI key   MUUHXGOJWVMBDY-UHFFFAOYSA-L

Description

Application

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Packaging

1, 5, 10, 25 g in glass bottle

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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