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T6399 Sigma-Aldrich

Trichloroacetic acid

ACS reagent, ≥99.0%

Synonym: TCA

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Properties

Related Categories Acids, Acids & Bases, Acids and Bases, Biochemicals and Reagents, Chemical Synthesis,
grade   ACS reagent
vapor density   <1 (vs air)
vapor pressure   1 mmHg ( 51 °C)
InChI Key   YNJBWRMUSHSURL-UHFFFAOYSA-N
assay   ≥99.0%
impurities   Substances darkened by sulfuric acid, passes test
  ≤0.01% insoluble matter
ign. residue   ≤0.03%
refractive index   n20/D 1.62(lit.)
bp   196 °C(lit.)
mp   54-58 °C(lit.)
solubility   H2O: 1 g/10 mL
density   1.62 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤0.002%
  nitrate (NO3-): ≤0.002%
  phosphate (PO43-): ≤5 ppm
  sulfate (SO42-): ≤0.02%
cation traces   Fe: ≤0.001%
  heavy metals (as Pb): ≤0.002%
storage temp.   2-8°C

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

• Trichloroacetic acid has been used for the precipitation of sLRP (soluble circulating low density lipoprotein receptor-related protein-1) isolated from plasma.
• It has been used for the extraction of ascorbic acid from plant samples. It has also been used for the preparation of ascorbate standards for ascorbate assay.
• It has been used for the precipitation of IMAC (immobilized metal ion affinity chromatography)-isolated phosphoproteins.

Packaging

1 kg in glass bottle

5, 100, 4×100, 250, 500 g in glass bottle

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1839 8 / PGII
WGK Germany 
2
RTECS 
AJ7875000
Flash Point(F) 
>235.4 °F
Flash Point(C) 
>113 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers
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References

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