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T9284 Sigma-Aldrich

Triton X-100

BioXtra

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Apoptosis and Cell Cycle, Biochemicals and Reagents, Cell Biology, Cell Culture,
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
product line   BioXtra
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
application(s)   permeability assay: suitable
impurities   ≤0.05% Phosphorus (P)
ign. residue   ≤0.4%
CMC   0.2-0.9 mM (20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: soluble
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.1%
  Pb: ≤0.001%
  Zn: ≤0.0005%
HLB   13.5

Description

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
• Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
• As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
• In the blocking solution during immunocytochemistry procedure
• During deglycosylation procedure
• For membrane solubilization
• For cell permeabilization
• In decellularization of porcine heart valve tissue

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Span is a registered trademark of Croda International PLC

TWEEN is a registered trademark of Croda International PLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
2
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Protocols

Immunofluorescence Labeling of Cells

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Microscopy, Solvents, Titrations

Peer-Reviewed Papers
15

References

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