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U1250 Sigma-Aldrich

Urea

ReagentPlus®, ≥99.5%, pellets

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 57654718

  •  NACRES NA.21

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Properties

Related Categories Amino Acid, Biochemicals and Reagents, Cell Biology, Chaotropic Agents, Denaturation,
Quality Level   300
grade   ReagentPlus®
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
assay   ≥99.5%
form   pellets
mp   132-135 °C (lit.)
solubility   H2O: 8 M
density   1.335 g/mL at 25 °C (lit.)
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.

Application

Urea has been used as a protein denaturing agent.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

1 kg in poly bottle

5 kg in poly drum

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

This procedure may be used for determination of Proteinase K activity using hemoglobin as the substrate. It is not used to assay the activity of immobilized Proteinase K products, such as Catalog Num...
Keywords: Immobilization

Enzymatic Assay of Urease from Jack Beans

The scope of this procedure is for all products that have a specification for the enzymatic determination of Urease, from Jack Beans. This assay is not to be used to determine the activity of Urease,...
Keywords: Indicators, Sonication, Titrations

Peer-Reviewed Papers
15

References

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