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VT000133 Supelco

Escherichia coli WDCM 00013 Vitroids

80-130 CFU mean value range, certified reference material, for microbiology

  •  NACRES NA.24



Related Categories Analytical Standards, Analytical/Chromatography, Bacteria Standards, Microbiological Standards, Microbiology,
Quality Level   300
grade   certified reference material
  for microbiology
Featured Industry   Environmental
Food and Beverages
storage temp.   −20°C
suitability   Escherichia coli


General description

This certified reference material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.
Download your certificate at: http://www.sigma-aldrich.com.

Please specify if you require a single batch - in the remark field when ordering (only for orders with more than 15 packs)

Vitroids discs contain Escherichia coli WDCM 00013 viable microorganisms (80-130 CFU) in a certified quantity (generally accredited according to ISO/IEC 17025), produced under reproducible conditions compliant with ISO 17034:2016 using authenticated strains from CECT®. The discs consist of pure cultures of bacteria in a solid matrix, stable for at least one year and are in a viable state with a shelf life of 1-3 years.
Each batch is provided with a comprehensive certificate of analysis that specifies the mean number of colony-forming units (CFU), an expanded uncertainty about the mean, details about the method used to determine the product data and the number of passages (subcultures) from the original strain.
Vitroids are derived from a traceable culture obtained freeze-dried from CECT®.


• Daily quality control
• Performance testing of media acc. ISO 11133
• Validation of new methods
• Materials for proficiency testing or ring trials
• Method development
• Staff training
• Starter cultures

Features and Benefits

• Defined CFU range and low standard deviation
• Fast, reliable and easy to use

Other Notes

ATCC® 25922, NCTC 12241, NCPF -, DSM 1103, LMG 8223, IHEM -, CIP 76.24

Origin Strain No. CECT® 434

Legal Information

Derived from CECT® strains

ATCC is a registered trademark of American Type Culture Collection

CECT is a registered trademark of Universitat de Valencia

Vitroids is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

UN3373 - class 6.2 Biological substance, Category B
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

USDA Interstate Transport Permit Process for Vitroids™ and Lenticule® Discs

Customers in the United States, who are located outside of Missouri (where the products are distributed), should apply for and obtain a USDA Transport Permit to receive Vitroids™ and Lenticule® Disc ...
Keywords: Cell culture, Veterinary

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