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X100 Sigma-Aldrich

Triton X-100

laboratory grade

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents,
grade   laboratory grade
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
CMC   0.2-0.9 mM (20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB   13.5

Description

General description

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

5 mL in glass bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Protocols

Enzymatic Assay of Cholesterol Oxidase

To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations

Immunoprecipitation Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

Solubilization of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

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