EMAIL THIS PAGE TO A FRIEND
X100 Sigma-Aldrich

Triton X-100

laboratory grade

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

Purchase

Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents,
grade   laboratory grade
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: 0.1 mL/mL clear to slightly hazy, colorless to faintly yellow
HLB   13.5

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

5 mL in glass bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability


LC-MS Grade Solvents and Reagents

Bulk Chemicals

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Frequently Asked Questions

What is the molecular weight of X100, Triton® X-100?
This information can be found on product information sheet for Product No. X100. Based on an average of 9-10 ethylene oxide units per molecule, the molecular weight of Triton X-100 is 625 g/mol. Here is the link to that document: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/x100pis.Par.0001.File.tmp/x100pis.pdf
What is the concentration of X100, Triton® X-100?
This product is 100% active ingredient. Here is the link to the product information sheet for this item: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/x100pis.Par.0001.File.tmp/x100pis.pdf
What concentration of X100,Triton® X-100, is typically used to lyse cells?
This information can be found on the X100 product information sheet. Typically, a 0.1 % solution of Triton X-100 is sufficient for lysing cells. Please click on the following link to view the document: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/x100pis.Par.0001.File.tmp/x100pis.pdf
Does X100, Triton® X-100, have any UV absorbance?
X100 does have UV absorbance at 280 nm, which will interfere with the UV determination of proteins.
Can I autoclave X100, Triton® X-100?
This information can be found on the product information sheet for Triton X-100.  Aqueous solutions of Triton X-100 are stable to autoclaving. At certain concentrations the solutions may be cloudy but dispersible above the cloud point. However, the solution should clear with stirring upon cooling. Here is the link to that document: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/x100pis.Par.0001.File.tmp/x100pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Detergent Properties and Applications

The key to detergent function is an amphipathic structure. All detergents are characterized as containing a hydrophilic “head” region and a hydrophobic “tail” region (see Figure 1).
Vicki Caligur
BioFiles 2008, 3.3, 14.
Keywords: Adsorption, Biochemistry, Cell disruption, Chromatography, Crystallization, Detergents, Diagnostic, Dialysis, Electrophoresis, Enzymology, Filtration, Mass spectrometry, Normal-phase chromatography, Nucleic acid denaturation, Precipitation, Purification, Separation, Size-exclusion chromatography

Protocols

Enzymatic Assay of Cholesterol Oxidase

To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations

Peer-Reviewed Papers
15

References

Related Products

also commonly purchased with this product

Product #

Description

Add to Cart

D9652 Dialysis tubing cellulose membrane, avg. flat width 33 mm (1.3 in.)
F1051 Fetal Bovine Serum, Canada origin, sterile-filtered, suitable for cell culture
56753 2-Phenoxyethanol, analytical standard
S9378 Sucrose, ≥99.5% (GC)
T5125 Thimerosal, ≥97% (HPLC)
P1379 TWEEN® 20, viscous liquid
P5648 β-Propiolactone, Grade II, ≥90%
T1503 Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
F8775 Formaldehyde solution, for molecular biology, 36.5-38% in H2O

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?