X100 Sigma-Aldrich

Triton X-100

laboratory grade

Synonym: t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

  • CAS Number 9002-93-1

  • Linear Formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10

  •  Beilstein/REAXYS Number 2315025

  •  MDL number MFCD00128254

  •  PubChem Substance ID 329830772

  •  NACRES NA.21



Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents,
Quality Level   200
grade   laboratory grade
description   non-ionic
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
CMC   0.2-0.9 mM (20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB   13.5
InChI   1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3


General description

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.


Triton-X has been used:
• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope


1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

5 mL in glass bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 3082 9 / PGIII
WGK Germany 
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

What is the molecular weight of X100, Triton® X-100?
Based on an average of 9-10 ethylene oxide units per molecule, the molecular weight of Triton X-100 is 625 g/mol.
What is the concentration ofX100, Triton® X-100?
This product is 100% active ingredient.
What concentration of X100,Triton® X-100, is typically used to lyse cells?
Typically, a 0.1 % solution of Triton X-100 is sufficient for lysing cells.
Does X100, Triton® X-100,have any UV absorbance?
X100 does have UV absorbance at 280 nm, which will interfere with the UV determination of proteins.
Can I autoclave X100, Triton® X-100?
Aqueous solutions of Triton X-100 are stable to autoclaving. At certain concentrations the solutions may be cloudy but dispersible above the cloud point. However, the solution should clear with stirring upon cooling.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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pHast Pack
Protocols & Articles


Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription


Enzymatic Assay of Cholesterol Oxidase

To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations

Immunoprecipitation Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

Solubilization of Membrane Proteins

This is one of the most critical stages during the preparation of membrane proteins. During the solubilization stage, membrane proteins are extracted from their natural environment, the lipid membran...
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

Peer-Reviewed Papers


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