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X100PC Sigma-Aldrich

Triton X-100

peroxide- and carbonyl-free

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents,
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
packaging   ampule of 5, 5x5, 10x5 mL
aggregation number   100-155
CMC   0.2-0.9 mM (20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: soluble
HLB   13.5

Description

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

0·3% Triton X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Packaging

5, 5×5, 10×5 mL in ampule

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

Packaged in ampules under Argon.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND

Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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