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X114 Sigma-Aldrich

Triton X-114

laboratory grade

Synonym: (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether

  • CAS Number 9036-19-5

  • Linear Formula (C2H4O)n C14H22O, n = 7 or 8

  •  Beilstein/REAXYS Number 2315025

  •  MDL number MFCD00128254

  •  PubChem Substance ID 24902129

  •  NACRES NA.21

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Properties

Related Categories Biochemicals and Reagents, Cell Culture, Cell Lysis and Protein Extraction Reagents, Core Bioreagents, Detergents,
Quality Level   200
grade   laboratory grade
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt   average mol wt 537
composition   t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8
refractive index   n20/D 1.488 (lit.)
CMC   0.2 mM (20-25°C)
transition temp   pour point ~−9 °C
  cloud point 23 °C
density   1.058 g/mL at 25 °C (lit.)
HLB   12.4

Description

General description

Triton X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton X-114 has been used:
• To remove endotoxin from solutions of protein using phase separation technique
• In the extraction and purification of DNA from endotoxin
• In the biphasic extraction of lipoproteins from bacterial cell wall

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

GPI Anchored Glycoproteins

Glycosylphosphatidylinisotol (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting they a...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Cancer, Glycosylations, Transduction

Peer-Reviewed Papers
15

References

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