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04276 Sigma-Aldrich

Sodium phosphate dibasic

puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)

Synonym: sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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Properties

Related Categories Chemical Synthesis, Inorganic Salts, Sodium, Synthetic Reagents
grade   puriss.
assay   98-100.5% (calc. to the dried substance)
quality   meets analytical specification of Ph. Eur., BP, USP, FCC, E 339
impurities   reducing matter, complies
  residual solvents, complies
  ≤0.001% heavy metals (as Pb)
  ≤0.002% ammonium (NH4)
  ≤0.2% water-insoluble matter
  ≤1% NaH2PO4 (calc. to dried subst.)
  ≤2.0% pyrophosphate (as Na4P2O7)
loss   ≤1% loss on drying, 105°C, 4h
  ≤1% loss on drying, 130 °C
pH   8.4-9.6 (20 °C, 1%)
pKa (25 °C)   (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
solubility   H2O: soluble 8 parts
  H2O: very soluble (Hot)
  alcohol: insoluble
anion traces   chloride (Cl-): ≤50 mg/kg
  fluoride (F-): ≤10 mg/kg
  sulfate (SO42-): ≤200 mg/kg
cation traces   As: ≤2 mg/kg
  Cd: ≤1 mg/kg
  Cu: ≤10 mg/kg
  Fe: ≤20 mg/kg
  Hg: ≤1 mg/kg
  Pb: ≤4 mg/kg
  Zn: ≤10 mg/kg
suitability   complies for appearance of solution
SMILES string   [Na+].[Na+].[H]OP([O-])([O-])=O
InChI   1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
InChI key   BNIILDVGGAEEIG-UHFFFAOYSA-L

Description

Application

Sodium phosphate dibasic has been used for preparing phosphate buffer which in turn is utilized in X-gal staining.

Biochem/physiol Actions

Sodium phosphate dibasic is an important component of running buffer of denaturing gel electrophoresis. It induces glomerular calcification as well as other nephrotic syndrome in Sprague-Dawley rats.

Safety & Documentation

Safety Information

WGK Germany 
1
RTECS 
WC4500000

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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