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302031 Sigma-Aldrich

Trifluoroacetic acid

for HPLC, ≥99.0%

Synonym: TFA

  • CAS Number 76-05-1

  • Linear Formula CF3COOH

  • Molecular Weight 114.02

  •  Beilstein/REAXYS Number 742035

  •  EC Number 200-929-3

  •  MDL number MFCD00004169

  •  PubChem Substance ID 329753348

  •  NACRES NA.21

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Properties

Related Categories Acids, Acids & Bases, Chemical Synthesis, Organic Acids, Synthetic Reagents More...
Quality Level   100
grade   for HPLC
vapor density   3.9 (vs air)
vapor pressure   97.5 mmHg ( 20 °C)
assay   ≥99.0%
impurities   <0.050% water
evapn. residue   <0.005%
refractive index   n20/D 1.3 (lit.)
bp   72.4 °C (lit.)
mp   −15.4 °C (lit.)
solubility   water: soluble
density   1.489 g/mL at 20 °C (lit.)
λ   H2O reference
UV absorption   λ: 260 nm Amax: 0.9
  λ: 270 nm Amax: 0.10
  λ: 280 nm Amax: 0.05
  λ: 290 nm Amax: 0.04
  λ: 300 nm Amax: 0.03
  λ: 320 nm Amax: 0.025
Featured Industry   Food and Beverages
SMILES string   OC(C(F)(F)F)=O
InChI   1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
InChI key   DTQVDTLACAAQTR-UHFFFAOYSA-N

Description

Packaging

1 L in glass bottle

100, 4×100 mL in glass bottle

10×1 mL in ampule

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2699 8 / PGI
WGK Germany 
2
RTECS 
AJ9625000
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Fast UHPLC Analysis of SILu™ Lite SigmaMAb Universal Antibody Standard IdeS Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents Fast UHPLC Analysis of SILu™ Lite SigmaMAb Universal Antibody Standard IdeS Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, Immobilization, Mass spectrometry, Reductions, Reversed-phase chromatography, Sample preparations, Separation

HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection
Keywords: Chromatography, Derivatizations, High performance liquid chromatography, Sample preparations, Solid phase extractions

HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea
Keywords: Agriculture, Chromatography, Derivatizations, High performance liquid chromatography, Metabolites, Sample preparations, Size-exclusion chromatography, Solid phase extractions

HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea
Keywords: Chromatography, Derivatizations, High performance liquid chromatography, Size-exclusion chromatography, Solid phase extractions

HPLC Analysis of Albuterol Enantiomers on Astec® CHIROBIOTIC® T2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Albuterol Enantiomers on Astec® CHIROBIOTIC® T2
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Alprenolol Enantiomers on Astec® CHIROBIOTIC® T2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Alprenolol Enantiomers on Astec® CHIROBIOTIC® T2
Keywords: Antihypertensives, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Benzoic Acid Derivatives on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzoic Acid Derivatives on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Benzoin Enantiomers on Astec® Cellulose DMP

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzoin Enantiomers on Astec® Cellulose DMP
Keywords: Antibiotics, Chromatography, High performance liquid chromatography

HPLC Analysis of Beta Blockers on Ascentis® Express C18 using a 60 Second Gradient

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Beta Blockers on Ascentis® Express C18 using a 60 Second Gradient
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxyamido-Methylated BSA Tryptic Digest on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamido-Methylated BSA Tryptic Digest on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C8

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C8
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxymethylated Cytochrome c (Chicken) Tryptic Digest on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Cytochrome c (Chicken) Tryptic Digest on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Caseins on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography, Separation

HPLC Analysis of Clenbuterol Enantiomers on Astec® CHIROBIOTIC® T2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Clenbuterol Enantiomers on Astec® CHIROBIOTIC® T2
Keywords: Chromatography, Clinical, Forensic, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Dansyl-Glutamic Acid Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Dansyl-Glutamic Acid Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Dansyl-Leucine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Dansyl-Leucine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Dansyl-Methionine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Dansyl-Methionine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Dansyl-Phenylalanine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Dansyl-Phenylalanine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Dansyl-Valine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Dansyl-Valine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Etodolac Enantiomers on Astec® Cellulose DMP

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Etodolac Enantiomers on Astec® Cellulose DMP
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of FMOC-Arginine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of FMOC-Arginine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of FMOC-Lysine Enantiomers on Astec® CHIROBIOTIC® R

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of FMOC-Lysine Enantiomers on Astec® CHIROBIOTIC® R
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Human Serum Albumin Tryptic Digest on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Human Serum Albumin Tryptic Digest on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Humira on BIOshell™ A400 Protein C4 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Humira on BIOshell™ A400 Protein C4 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C8

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C8
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide CN

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide CN
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery® BIO Wide Pore C5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery® BIO Wide Pore C5
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Methorphan Enantiomers on Astec® CHIROBIOTIC® V2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Methorphan Enantiomers on Astec® CHIROBIOTIC® V2
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Metoprolol Enantiomers on Astec® CHIROBIOTIC® T2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Metoprolol Enantiomers on Astec® CHIROBIOTIC® T2
Keywords: Antihypertensives, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Monoclonal Antibody Heavy and Light Chains on Zenix® SEC-300

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Monoclonal Antibody Heavy and Light Chains on Zenix® SEC-300
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column
Keywords: Chromatography, Genetic, High performance liquid chromatography, Oxidations

HPLC Analysis of Nebivolol Enantiomers on Astec® CHIROBIOTIC® V2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Nebivolol Enantiomers on Astec® CHIROBIOTIC® V2
Keywords: Antihypertensives, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Organic Acids on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Organic Acids on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Organic Acids on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Organic Acids on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on BIOshell™ A160 Peptide C18 and CN

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on BIOshell™ A160 Peptide C18 and CN
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V
Keywords: Chromatography, High performance liquid chromatography, Metabolites, Separation

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18
Keywords: High performance liquid chromatography

HPLC Analysis of Reduced Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Reduced Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reductions

HPLC Analysis of SILu Lite Antibody Standard on BIOshell™ A400 Protein C4 Versus Competitor Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of SILu Lite Antibody Standard on BIOshell™ A400 Protein C4 Versus Competitor Column
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm
Keywords: Alkylations, Chromatography, High performance liquid chromatography, Nucleic acid denaturation, Sample preparations

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease
Keywords: Chromatography, Digestions, High performance liquid chromatography, Separation, Size-exclusion chromatography

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of α-Casein Tryptic Digest on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of α-Casein Tryptic Digest on BIOshell™ A160 Peptide C18
Keywords: Chromatography, Detection methods, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Proteomics, Separation

HPLC of monoclonal antibody (mAb) SigmaMab on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC of monoclonal antibody (mAb) SigmaMab on BIOshell™ A400 Protein C4
Keywords: High performance liquid chromatography, Separation

LC/MS Analysis of β-Lactoglobulin Tryptic Digest on a Discovery® BIO Wide Pore C18 Capillary Column

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of β-Lactoglobulin Tryptic Digest on a Discovery® BIO Wide Pore C18 Capillary Column
Keywords: Chromatography, High performance liquid chromatography

Reversed-Phase HPLC Analysis of Insulin Variants and Analogs by UV and Mass Spectral Detection

Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recomb...
Cory Muraco, Stacy Shollenberger, and Hillel Brandes
MilliporeSigma, Bellefonte, PA USA
Keywords: Chromatography, High performance liquid chromatography, Hormones, Mass spectrometry, Pharmaceutical, Phase transitions, Reversed-phase chromatography, Separation

TLC Analysis Of A Natural Product From Cosmetical Matrix on HPTLC Silica gel 60 NH2 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis Of A Natural Product From Cosmetical Matrix on HPTLC Silica gel 60 NH2 F254s
Keywords: Gene expression, Mass spectrometry, Sample preparations, Thin layer chromatography

TLC Analysis Of Caffeine in energy drinks on HPTLC Silica gel 60 F254 MS-grade

From our library of Articles, Sigma-Aldrich presents TLC Analysis Of Caffeine in energy drinks on HPTLC Silica gel 60 F254 MS-grade
Keywords: Gene expression, Mass spectrometry, Sample preparations, Thin layer chromatography

TLC Analysis Of Compounds in Amelanchir Ovalis Extract on HPTLC Silica gel 60 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis Of Compounds in Amelanchir Ovalis Extract on HPTLC Silica gel 60 F254s

TLC Analysis Of Stevioside and Rebaudioside in Stevia Extract on HPTLC Silica Gel 60 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis Of Stevioside and Rebaudioside in Stevia Extract on HPTLC Silica Gel 60 F254s
Keywords: Filtration, Mass spectrometry, Sample preparations, Thin layer chromatography

UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection
Keywords: Chromatography, Derivatizations, Sample preparations

USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

From our library of Articles, Sigma-Aldrich presents USP HPLC Analysis of L-Methionine on Ascentis® Express OH5
Keywords: Chromatography, High performance liquid chromatography

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