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31437 Sigma-Aldrich

Sodium bicarbonate

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.7%

Synonym: Sodium hydrogen carbonate

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Properties

Related Categories Analytical Reagents, Analytical Reagents for General Use, Analytical/Chromatography, Essential Chemicals, Puriss p.a. ACS,
grade   ACS reagent, reag. Ph. Eur.
InChI Key   UIIMBOGNXHQVGW-UHFFFAOYSA-M
grade   puriss. p.a.
assay   ≥99.7%
impurities   ≤0.0005% ammonium (NH4)
  ≤0.003% S-compounds (as SO4)
  ≤0.005% I red. matters (as SO2)
  ≤0.015% in water insoluble matters
pH   ≤8.6 (20 °C, 5%)
pKa (25 °C)   (1) 6.37, (2) 10.25 (carbonic acid)
solubility   H2O: 0.5 g/10 mL, clear, colorless
  water: soluble 50 g/L
anion traces   chloride (Cl-): ≤10 mg/kg
  phosphate (PO43-): ≤10 mg/kg
cation traces   As: 2 ppm (MHS-AAS)
  Ca: ≤100 ppm
  Cd: ≤5 ppm
  Co: ≤50 ppm
  Cu: ≤5 ppm
  Fe: ≤5 ppm
  K: ≤50 ppm
  Mg: ≤50 ppm
  Ni: ≤50 ppm
  Pb: ≤5 ppm
  Zn: ≤5 ppm
  heavy metals: ≤5 ppm (by ICP-OES)
suitability   complies for appearance of solution

Description

Application

Sodium bicarbonate may be used to maintain 7.4pH in Iscove′s modified Dulbecco′s medium for growth of murine hybridoma cell culture. It may be employed as a component of RPMI-1640 liquid medium in a study.

General description

Sodium bicarbonate is widely used as a physiological buffer. The pKa for bicarbonate ↔ carbonic acid reaction is 6.37. The pKa for bicarbonate ↔ carbonate reaction is 10.25.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
VZ0950000
Protocols & Articles
Peer-Reviewed Papers
15

References

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