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34854 Sigma-Aldrich

Chloroform

for HPLC, ≥99.8%, amylene stabilized

Synonym: Methylidyne trichloride, Trichloromethane

  • CAS Number 67-66-3

  • Empirical Formula (Hill Notation) CHCl3

  • Molecular Weight 119.38

  •  Beilstein/REAXYS Number 1731042

  •  EC Number 200-663-8

  •  MDL number MFCD00000826

  •  PubChem Substance ID 329755063

  •  NACRES NA.04

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Properties

Related Categories Amber Glass Bottles, Analytical Reagents, Analytical/Chromatography, Chloroform, Chromatography Reagents & Solvents,
grade   for HPLC
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
assay   ≥99.8%
quality   amylene stabilized
impurities   Free acid, in accordance
  acetone, aldehyde, in accordance (nessler test)
  free chlorine (Cl), in accordance
  ≤0.0005% non-volatile matter
  ≤0.001% free acid (as HCl)
  <0.01% water (Karl Fischer)
evapn. residue   ≤0.0005%
color   APHA: ≤10
transmittance   250 nm, ≥50%
  260 nm, ≥85%
  280 nm, ≥99%
refractive index   n20/D 1.445 (lit.)
bp   60.5-61.5 °C (lit.)
mp   −63 °C (lit.)
density   1.48 g/mL at 25 °C
  1.492 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): in accordance
cation traces   Pb: ≤0.05 mg/kg
absorption   in accordance
λ   H2O reference
UV absorption   λ: 250 nm Amax: ≤1.00
  λ: 260 nm Amax: ≤0.30
  λ: 280 nm Amax: ≤0.005
suitability   passes test for reaction against H2SO4
  passes test for suitability of determ. w. dithizone
Featured Industry   Food and Beverages
SMILES string   ClC(Cl)Cl
InChI   1S/CHCl3/c2-1(3)4/h1H
InChI key   HEDRZPFGACZZDS-UHFFFAOYSA-N

Description

General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Application

Chloroform has been employed for the ion exchange isolation of sulfatides from the urinary samples by using DEAE-cellulose membranes before the tandem mass spectrometric analysis. It has been used as a solvent for the Langmuir-Blodgett (LB) deposition of lipid bilayers over mica sheets (silver coated back).†

Packaging

1, 4×4 L in glass bottle

100 mL in glass bottle

Other Notes

Important notice
• The article number 34854-4X2.5L will be discontinued. Please order the single bottle 34854-2.5L which is physically identical with the same exact specifications.
• The article number 34854-4X2.5L-M will be discontinued. Please order the single bottle 34854-2.5L-M which is physically identical with the same exact specifications.
• The article number 34854-6X1L-M will be discontinued. Please order the single bottle 34854-1L-M which is physically identical with the same exact specifications.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
3
RTECS 
FS9100000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Dispensette™ S
Protocols & Articles

Articles

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Peer-Reviewed Papers
15

References

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