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34860 Sigma-Aldrich

Methanol

for HPLC, ≥99.9%

Synonym: Methyl alcohol

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Properties

Related Categories Amber Glass Bottles, Analytical Reagents, Analytical/Chromatography, Chromatography Reagents & Solvents, HPLC,
grade   for HPLC
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
InChI Key   OKKJLVBELUTLKV-UHFFFAOYSA-N
assay   ≥99.9%
autoignition temp.   725 °F
expl. lim.   36 %
impurities   ≤0.0005% non-volatile matter
  ≤1 ppb fluorescence (quinine) at 254 nm
  ≤1 ppb fluorescence (quinine) at 365 nm
  <0.03% water (Karl Fischer)
evapn. residue   <0.0005%
color   APHA: ≤10
refractive index   n20/D 1.329(lit.)
bp   64.7 °C(lit.)
mp   −98 °C (lit.)
density   0.791 g/mL at 25 °C(lit.)
λ   neat
UV absorption   λ: 205 nm Amax: ≤1.00
  λ: 210 nm Amax: ≤0.60
  λ: 220 nm Amax: ≤0.30
  λ: 230 nm Amax: ≤0.20
  λ: 235 nm Amax: ≤0.10
  λ: 240 nm Amax: ≤0.10
  λ: 260 nm Amax: ≤0.04
  λ: 280 nm Amax: ≤0.01
  λ: 400 nm Amax: ≤0.01

Description

Packaging

1, 6×1, 2, 4×2, 4, 4×4 L in glass bottle

18 L in Pure-Pac™ 1

View returnable container options.

100 mL in glass bottle

20, 50, 200 L in Pure-Pac™ 2

Preparation Note

Product filtered through a 0.2 μm filter

Application

Methanol (MeOH) has been used in the wholemount immunofluorescence studies of embryonic tissues.
It may be used in the following studies:
• Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
• As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
• Immunofluorescence studies.
• To compose the eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.

Methanol can be used as a mobile phase in reversed-phase liquid chromatography.

General description

Methanol is an organic solvent that can be synthesized from syngas in the presence of CuO/ZnO/Al2O3 catalysts. It is an ideal candidate as a hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and as it exists in liquid state at room temperature. In a direct methanol fuel cell (DMFC), methanol undergoes oxidation with air to generate electricity. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternate to oil and gas to produce hydrocarbon fuels.

Thermochemical conversion of methanol to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

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Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
1
RTECS 
PC1400000
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Who do I contact about larger solvent volume needs?
Email us at labessentials@sial.com or visit Sigma-Aldrich Fine Chemicals for development and manufacturing-scale inquiries: safcglobal@sial.com
How do I know what packaging options are available for a solvent?
The Solvent Center gives you the ability to review all the specifications associated with our solvent packaging. This includes physical dimensions, closure types, dispensing methods, UN/DOT Rating as well as solvent compatibility. Visit the Solvent Center.
Who do I contact if I need a solvent blend, which you do not carry?
Email us at labessentials@sial.com. For a complete listing of all our Solvent blends, visit the Solvent Center
The dispensing of solvents provides some challenges due to the number of container options available. What dispensing options are available for each container?
The Sigma-Aldrich Solvent Center provides numerous dispensing options for each container type. For a complete listing of all our dispensing options, visit the Solvent Center.
Which solvent grade should I be using for my application?
Sigma-Aldrich Solvent Applications Table gives a guideline to common applications and the appropriate solvent grades. Visit:Solvents
What are the options for drying solvents?
Sigma-Aldrich offers a full range of high-purity solvents with extremely low water levels specifically manufactured for moisture sensitive Organic and Biotech applications. Sigma-Aldrich also carries various drying agents such as molecular sieves, which are typically compatible with organic solvents.
What apparatus do you recommend for HPLC solvent filtration?
The Sigma-Aldrich Vacuum Filtration Assembly gives you all the components you need including: funnel top, fritted glass funnel support, filtration flask, aluminum clamp, and silicone stopper.The Acrodisc Syringe Filters offer high quality filtration for analytical samples, certified for HPLC to ensure low extractable and available in a broad range of membranes to meet sample compatibility requirements.
Can you recommend a Solvent Extractor for organics from aqueous solution?
Solvent extractors are often used for the quantitative extraction of organics from aqueous solutions and separations in immunoassays. The MIXXOR system has been applied successfully in many laboratory solvent extraction operations and is ideal for the rapid screening of alternative solvents for specific extraction problems.
Why on occasion doesProduct 34860,Methanol, have an unusual odor? Does it affect the product purity or performance?
Industry research has indicated that unusual odors in methanol are a common problem and stem from low - level mercaptans and / or amines found in the natural gas raw materials used in production. Odors from these types of compounds can be noticeable at low part per million (ppm) levels and have not been shown to have any negative impact on product performance at these concentrations.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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PTFE Millex filter
Protocols & Articles

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Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Protocols

Glycome mapping on DNA sequencing equipment

a Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, Ghent University, and VIB, Technologiepark 927
Keywords: Fractionation, High performance liquid chromatography, Mass spectrometry, Molecular biology, Sequencing

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Use the interactive controls above to simplify calculations and improve the efficiency of your distillation or evaporation requirements.
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Properties of Solvents Table

The table below presents the physical characteristics of common solvents. Easily reference or compare solvent properties like boiling point, density, and refractive index all from one list.
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Peer-Reviewed Papers
15

References

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