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34885 Sigma-Aldrich

Methanol

for HPLC, gradient grade, ≥99.9%

Synonym: Methyl alcohol

  • CAS Number 67-56-1

  • Linear Formula CH3OH

  • Molecular Weight 32.04

  •  Beilstein/REAXYS Number 1098229

  •  EC Number 200-659-6

  •  MDL number MFCD00004595

  •  PubChem Substance ID 329755085

  •  NACRES NA.05

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Properties

Related Categories Amber Glass Bottles, Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents, Chromatography Reagents & Solvents,
Quality Level   100
grade   for HPLC
  gradient grade
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
assay   ≥99.9%
form   liquid
autoignition temp.   725 °F
expl. lim.   36 %
application(s)   immunofluorescence: suitable
impurities   KMnO4 red. matter (as O), in accordance
  ≤0.0005% free alkali (as NH3)
  ≤0.0005% non-volatile matter
  ≤0.001% acetaldehyde
  ≤0.001% acetone (GC)
  ≤0.001% formaldehyde
  ≤0.02% water (Karl Fischer)
  ≤1 ppb fluorescence (quinine) at 254 nm
  ≤1 ppb fluorescence (quinine) at 365nm
evapn. residue   <0.0003%
color   APHA: ≤10
refractive index   n20/D 1.329 (lit.)
bp   64.7 °C (lit.)
mp   −98 °C (lit.)
density   0.791 g/mL at 25 °C (lit.)
HPLC-gradient   ≤2 mAU at 230 nm
  ≤5 mAU at 254 nm
absorption   in accordance
UV absorption   λ: 210 nm Amax: 0.50
  λ: 220 nm Amax: 0.30
  λ: 230 nm Amax: 0.15
  λ: 235 nm Amax: 0.10
  λ: 240 nm Amax: 0.05
  λ: 260 nm Amax: 0.01
  λ: 400 nm Amax: 0.01
Featured Industry   Food and Beverages
SMILES string   CO
InChI   1S/CH4O/c1-2/h2H,1H3
InChI key   OKKJLVBELUTLKV-UHFFFAOYSA-N

Description

General description

Thermochemical conversion of methanol to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

Application

Methanol (MeOH) has been used as an eluent in the HPLC estimation of malondialdehyde in plasma, which is an indicator of oxidative stress.
It may be used in the following studies:
• Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
• As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
• Immunofluorescence studies.
• To compose eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.

Packaging

1 pkg in Pure-Pac™ 2

1, 6×1, 2, 4, 4×4 L in glass bottle

100 mL in glass bottle

View returnable container options.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.

Important notice
• The article number 34885-4X2.5L-R will be discontinued. Please order the single bottle 34885-2.5L-R which is physically identical with the same exact specifications.

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Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Eyes
RIDADR 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
WGK 2
RTECS 
PC1400000
Flash Point(F) 
49.5 °F - closed cup
Flash Point(C) 
9.7 °C - closed cup

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Human Serum Albumin Tryptic Digest on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Human Serum Albumin Tryptic Digest on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Tetracycline Antibiotics on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tetracycline Antibiotics on Ascentis® Express C18
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Metabolites, Separation

LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

UHPLC/MS Analysis of NBOMe Designer Drugs in Urine on Ascentis® Express C18 after Solid Phase Extraction (SPE) using Supel™-Select HLB

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of NBOMe Designer Drugs in Urine on Ascentis® Express C18 after Solid Phase Extraction (SPE) using Supel™-Select HLB
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions, Solvents

Peer-Reviewed Papers
15

References

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