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47670 Sigma-Aldrich

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

Synonym: Amide C1, Formic amide

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Properties

Related Categories ACS Grade, Amber Glass Bottles, Analytical Reagents, Analytical Reagents for General Use, Analytical/Chromatography,
Quality Level   200
grade   ACS reagent
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
grade   puriss. p.a.
assay   ≥99.5% (GC/T)
autoignition temp.   932 °F
expl. lim.   2.7-19 %
impurities   ≤0.02% free acid (as HCOOH)
  ≤0.1% water
refractive index   n20/D 1.447 (lit.)
  n20/D 1.447
bp   210 °C (lit.)
mp   2-3 °C (lit.)
transition temp   transition temp 2.0-3.0 °C
density   1.132 g/mL at 20 °C
  1.134 g/mL at 25 °C (lit.)
cation traces   Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Zn: ≤1 mg/kg
suitability   in accordance for miscibility
SMILES string   NC=O
InChI   1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key   ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H2N-C group exhibits a shallow pyramid like structure.

Packaging

1, 2.5 L in glass bottle

4×25, 250 mL in glass bottle

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Blood
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
LQ0525000
Flash Point(F) 
347.0 °F
Flash Point(C) 
175 °C

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

GC Analysis of Class 2 Residual Solvents on OVI-G43

From our library of Articles, Sigma-Aldrich presents GC Analysis of Class 2 Residual Solvents on OVI-G43
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Pharmaceutical, Size-exclusion chromatography

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Peer-Reviewed Papers
15

References

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