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494437 Sigma-Aldrich

Methanol

BioReagent, ≥99.93%

Synonym: Methyl alcohol

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Properties

Related Categories Apoptosis and Cell Cycle, Biotech Solvents, Cell Biology, Cell Signaling and Neuroscience, Core Bioreagents,
grade   BioReagent
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
InChI Key   OKKJLVBELUTLKV-UHFFFAOYSA-N
assay   ≥99.93%
autoignition temp.   725 °F
expl. lim.   36 %
gradient analysis   ≤0.005, 254nm
impurities   ≤0.0002 meq/g Titr. base
  ≤0.0003 meq/g Titr. acid
  ≤0.02% (water)
evapn. residue   <0.0003%
color   APHA: <10
refractive index   n20/D 1.329(lit.)
bp   64.7 °C(lit.)
mp   −98 °C (lit.)
solubility   benzene: miscible(lit.)
  ethanol: miscible(lit.)
  water: miscible(lit.)
density   0.791 g/mL at 25 °C(lit.)
λ   H2O reference
UV absorption   λ: 203 nm Amax: 1.0
  λ: 210 nm Amax: 0.60
  λ: 220 nm Amax: 0.30
  λ: 230 nm Amax: 0.15
  λ: 240 nm Amax: 0.05
  λ: 260-400 nm Amax: 0.01

Description

Packaging

1, 2 L in Sure/Seal™

Preparation Note

Product filtered through a 0.2 μm filter

Application

Methanol has been used to fix resolved protein bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel.

General description

Methanol (MeOH) is an organic solvent with a wide range of industrial applications. It is the main component of lithium batteries. It can be prepared by hydrogenation of CO2 under various conditions. Shape-selective zeolite-assisted transformation of methanol to C2-C10 hydrocarbons has been studied. MeOH is a toxic alcohol employed for denaturing ethyl alcohol. A study reports the preparation of biodiesel from canola oil using methanol under supercritical conditions. Low water content, minimal residue and clean UV spectra are the special characteristics of this solvent. It can be utilized for biotechnological processes like protein sequencing, peptide and oligonucleotide synthesis.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
1
RTECS 
PC1400000
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

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Pressure-Temperature Nomograph Interactive Tool

Use the interactive controls above to simplify calculations and improve the efficiency of your distillation or evaporation requirements.
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Properties of Solvents Table

The table below presents the physical characteristics of common solvents. Easily reference or compare solvent properties like boiling point, density, and refractive index all from one list.
Keywords: Gas chromatography, Green chemistry, High performance liquid chromatography, Melting, Solvents

Peer-Reviewed Papers
15

References

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