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496189 Sigma-Aldrich

Chloroform

biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Synonym: Methylidyne trichloride, Trichloromethane

  • CAS Number 67-66-3

  • Empirical Formula (Hill Notation) CHCl3

  • Molecular Weight 119.38

  •  Beilstein/REAXYS Number 1731042

  •  MDL number MFCD00000826

  •  PubChem Substance ID 329757487

  •  NACRES NA.04

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Properties

Related Categories Biotech, Biotech Solvents, Chloroform, Core Bioreagents, General Reagents,
Quality Level   100
grade   biotech. grade
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
assay   ≥99.8%
form   liquid
contains   0.5-1.0% ethanol as stabilizer
impurities   <0.01% water
evapn. residue   <0.0003%
color   APHA: ≤10
refractive index   n20/D 1.445 (lit.)
pH   5.2-6.4
bp   60.5-61.5 °C (lit.)
mp   −63 °C (lit.)
density   1.48 g/mL at 25 °C
  1.492 g/mL at 25 °C (lit.)
cation traces   Pb: ≤0.05 ppm
SMILES string   ClC(Cl)Cl
InChI   1S/CHCl3/c2-1(3)4/h1H
InChI key   HEDRZPFGACZZDS-UHFFFAOYSA-N

Description

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Low water content, minimal residue and clean UV spectra are the special characteristics of this biotech grade solvent. It can be utilized for biotechnological processes like protein sequencing, peptide and oligonucleotide synthesis. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

Application

Chloroform may be used in the following processes:
• Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).
• Preparing phospholipid solutions.
• As a solvent in the photolysis reaction of ruthenium complexes.
• Solvent for recrystallization.
• Reference compound to detect the presence of chloroform in Tobacco smokes.
• Extractant in solvent extraction process.

Packaging

1, 2 L in Sure/Seal™

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Central nervous system
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Peer-Reviewed Papers
15

References

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