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496189 Sigma-Aldrich

Chloroform

biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Synonym: Methylidyne trichloride, Trichloromethane

  • CAS Number 67-66-3

  • Empirical Formula (Hill Notation) CHCl3

  • Molecular Weight 119.38

  •  Beilstein Registry Number 1731042

  •  MDL number MFCD00000826

  •  PubChem Substance ID 329757487

Purchase

Properties

Related Categories Biotech, Biotech Solvents, Chloroform, Core Bioreagents, General Reagents,
grade   biotech. grade
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
InChI Key   HEDRZPFGACZZDS-UHFFFAOYSA-N
assay   ≥99.8%
contains   0.5-1.0% ethanol as stabilizer
impurities   <0.01% water
evapn. residue   <0.0003%
color   APHA: ≤10
refractive index   n20/D 1.445(lit.)
pH   5.2-6.4
bp   60.5-61.5 °C(lit.)
mp   −63 °C (lit.)
density   1.48 g/mL at 25 °C
  1.492 g/mL at 25 °C(lit.)
cation traces   Pb: ≤0.05 ppm

Description

Packaging

1, 2 L in Sure/Seal™

Application

Chloroform may be used in the following processes:
• Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).
• Preparing phospholipid solutions.
• As a solvent in the photolysis reaction of ruthenium complexes.
• Solvent for recrystallization.
• Reference compound to detect the presence of chloroform in Tobacco smokes.
• Extractant in solvent extraction process.

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Low water content, minimal residue and clean UV spectra are the special characteristics of this biotech grade solvent. It can be utilized for biotechnological processes like protein sequencing, peptide and oligonucleotide synthesis. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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