650552 Sigma-Aldrich


HPLC Plus, for HPLC, GC, and residue analysis, ≥95%

Synonym: n-Hexane



Related Categories Amber Glass Bottles, Analytical Reagents, Analytical/Chromatography, Chromatography Reagents & Solvents, HPLC Plus,
grade   HPLC Plus
  for GC
  for HPLC
  for residue analysis
vapor density   ~3 (vs air)
vapor pressure   256 mmHg ( 37.7 °C)
  5.2 psi ( 37.7 °C)
  ~132 mmHg ( 20 °C)
assay   ≥95%
autoignition temp.   453 °F
expl. lim.   7.7 %
impurities   ≤ 1.0 ppb Fluorescence (quinine) at 365 nm
  <0.01% water
evapn. residue   <0.0001%
halogenated residue   <10 ng/L (as heptachlor epoxide)
refractive index   n20/D 1.375 (lit.)
bp   69 °C (lit.)
mp   −95 °C (lit.)
solubility   ethanol: soluble(lit.)
density   0.659 g/mL at 25 °C (lit.)
UV absorption   λ: 195 nm Amax: 1.00
  λ: 200 nm Amax: 0.50
  λ: 210 nm Amax: 0.20
  λ: 220 nm Amax: 0.05
  λ: 250 nm Amax: 0.01
  λ: 280-400 nm Amax: 0.005
Featured Industry   Food and Beverages


General description

Hexane (n-hexane) is an alkane, that can be obtained from petroleum by distillation. It can also be produced by electrolyzing the potassium butyrate solution. It is widely employed organic solvent for the dissolution and isolation of fats and oils. Its thermodynamic properties and kinetics of thermal decomposition have been studied over a range of temperature. A study on the zeolite supported platinum catalyzed isomerization and aromatization of n-hexane has been reported. Its removal from hexane contaminated air streams using vapor phase biological reactor (VPBR) immobilized with hexane-degrading Aspergillus niger strain has been studied. The metabolism of n-hexane forms 2,5-hexanedione (2,5-HD), which is reported to induce neurotoxicity.


Hexane may be used in the following studies:
• Preparation of graft-copolymer chains of poly-(dimethylsiloxane)-g-poly(methyl methacrylate).
• GC/MS characterization of essential oils isolated from various origins.
• As solvent for the preparation of samples and standards during chromatographic studies.
• To extract free and total gossypol in combination with acetone from cottonseed flakes.

Suitable for HPLC, spectrophotometry, environmental testing


1, 6×1, 4, 4×4 L in glass bottle

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Safety & Documentation

Safety Information

Signal word 
Precautionary statements 
UN1208 - class 3 - PG 2 - Hexanes
WGK Germany 
Flash Point(F) 
-14.8 °F
Flash Point(C) 
-26 °C


Certificate of Analysis


Certificate of Origin


Dispensette™ S
Protocols & Articles


Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

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Pressure-Temperature Nomograph Interactive Tool

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Properties of Solvents Table

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Peer-Reviewed Papers


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