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01494 Sigma-Aldrich

Nancy-520

Green Alternative

suitable for RT-PCR, ≥97.0% (HPCE)

Purchase

Description

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

WGK Germany 
2
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Frequently Asked Questions

How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the mutagenic potential of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?
The Ames test (II) has confirmed that Product 01494, Nancy-520, is less mutagenic than SYBR green I and ethidium bromide.
What is the sensitivity of Product 01494, Nancy-520,compared to SYBR Green I and ethidium bromide?
Product 01494, Nancy-520, shows a higher sensitivity than ethidium bromide and a slightly higher sensitivity to SYBR Green I.
Does Product 01494, Nancy-520,require the same safety handling that is required for Ethidium bromide?
No, the handling of Product 01494, Nancy-520, does not requirethe strict safety instructions that are needed for Ethidium bromide. Normal latex gloved may be used. No specific "Nitrile" gloves are required. No particularly equipped fume hood for Nancy-520 is required. Other work can be done in the same fume hood, where Nancy-520 is used.
Can Product 01494, Nancy-520 staining solution, be reused?
Re-use of Product 01494, Nancy-520 staining solution, is not recommended.
Can Nancy-520 be detected when using the Odyssey® Fc imager?
According to an application note published by LI-COR "Imaging Nucleic Acid Gels on the Odyssey® Fc Imager," published in 2011, Nancy-520 wassuccessfully tested in the Fc imager,using the 600 channel.®Odyssey is a registered Trademark of LI-COR, Inc. The trademark belongs to their respective owners. The Odyssey® Fc Imaging system is covered by U.S. patents, foreign equivalents, and patents pending.
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Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Quantitative PCR - PCR Technologies Guide

As described in Introduction and Historical Timelines and Polymerase Chain Reaction, PCR is currently considered to be the most useful technique that is applied to investigations using molecular biol...
Keywords: Amplification, Buffers, Clinical, Clinical Chemistry, Detection methods, Electrophoresis, Enzyme activity, Gel electrophoresis, Gene expression, Melting, Methylations, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, PAGE, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Size-exclusion chromatography, Transcription

Real-time PCR Study Report on Nancy-520

Sigma-Aldrich offers a fluorescent stain for dsDNA called Nancy-520, a greener alternative to Ethidium bromide. This dye has also been successful in PCR (Real time-PCR) applications. In order to deve...
Keywords: Amplification, Centrifugation, Electrophoresis, Gel electrophoresis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Titrations

Related Content

Greener Alternatives Three Categories

A new icon has been added to identify our Greener alternative products. Products with this icon fulfill one of the three criteria.
Keywords: Alternative energy, Environmental, Green chemistry, PAGE

Nancy-520 – The Safer, Stable Alternative to Ethidium Bromide and SYBR® Green

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was designed as a safer chemical. Ethidium bromide i...
Keywords: Catalytic combustion detector, Electrophoresis, Mutagens, PAGE, Substitutions

Peer-Reviewed Papers
15

References

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Description

Add to Cart

08168 Timestrip Plus 8 °C
E7637 Ethidium bromide, BioReagent, for molecular biology, powder

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