03285 Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

  •  eCl@ss 32161000

  •  NACRES NA.32



Related Categories Biochemicals and Reagents, Cell Culture, Cell Viability, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains,
Quality Level   100
usage   sufficient for 500 tests
storage temp.   −20°C



The Cell Counting Kit (CCK) is utilized for the fluorometric detection of living cell numbers. The amount of a fluorescent dye, calcein, produced from calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis[N,N-bis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lypohilic because of the acetoxymethy groups in the molecule, it can rapidly permeate into the cytoplasm through the cell membrane. The CCK assay does not require any radioisotopes (such as in the [3H]- thymidine incorporated assay) or a solubilization procedure (such as in the MTT assay). Therefore, it allows the users to obtain highly reproducible and accurate cell proliferation assay results.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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