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03285 Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

  •  eCl@ss 32161000

  •  NACRES NA.32

Purchase

Properties

Related Categories Biochemicals and Reagents, Cell Culture, Cell Viability, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains,
Quality Level   100
usage   sufficient for 500 tests
storage temp.   −20°C

Description

Application

The Cell Counting Kit (CCK) is utilized for the fluorometric detection of living cell numbers. The amount of a fluorescent dye, calcein, produced from calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis[N,N-bis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lypohilic because of the acetoxymethy groups in the molecule, it can rapidly permeate into the cytoplasm through the cell membrane. The CCK assay does not require any radioisotopes (such as in the [3H]- thymidine incorporated assay) or a solubilization procedure (such as in the MTT assay). Therefore, it allows the users to obtain highly reproducible and accurate cell proliferation assay results.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Keywords: Cancer, Cell culture, Cell disruption, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Indicators, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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