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03978 Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Synonym: Ehrlich’s reagent III, Loeffler’s Methylene Blue, Methylene blue stain

  • CAS Number 61-73-4

  • Empirical Formula (Hill Notation) C16H18ClN3S

  • Molecular Weight 319.85

  •  Beilstein/REAXYS Number 3641570

  •  MDL number MFCD00012111

  •  PubChem Substance ID 329747853

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   100
grade   for microscopy
form   liquid
quality   concentrated, aqueous solution
shelf life   ~3 yr
concentration   1.5% (1.5 g/ 100 mL)
application(s)   hematology: suitable
  histology: suitable
density   1.0 g/mL at 20 °C
εmax   9.4 at 656-666 nm in water
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI   1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
InChI key   CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright’s stain and Jenner’s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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