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04511 Sigma

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonym: Staining kit for live/dead cells

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Description

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

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CellASIC® ONIX2 System

Flow Cytometry

All labs need water

Kit component only

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Solution A (Calcein AM solution) 4 × 50 μL SDS    
Solution B (propidium iodide solution) 300 μL SDS    
Safety & Documentation

Safety Information

Flash Point(F) 
185 °F
Flash Point(C) 
85 °C
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Peer-Reviewed Papers
15

References

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