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07985 Sigma-Aldrich

6-Aminofluorescein

BioReagent, suitable for fluorescence, ~95% (HPLC)

Synonym: Fluoresceinamine isomer II

  • CAS Number 51649-83-3

  • Empirical Formula (Hill Notation) C20H13NO5

  • Molecular Weight 347.32

  •  Beilstein/REAXYS Number 51708

  •  EC Number 257-334-7

  •  MDL number MFCD00005051

  •  PubChem Substance ID 329748535

  •  NACRES NA.32

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Carbohydrate Labels, Carbohydrate Labels for Chromatography, Derivatization agents,
Quality Level   100
product line   BioReagent
assay   ~95% (HPLC)
impurities   ≤5% 5-aminofluorescein
mp   285 °C (dec.) (lit.)
solubility   DMSO: soluble
  acetone: soluble
  methanol: soluble
fluorescence   λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
suitability   suitable for fluorescence
SMILES string   Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
InChI   1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
InChI key   YOAWSYSKQHLFPM-UHFFFAOYSA-N

Description

Application

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes’. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.

Other Notes

Note: Do not confuse with fluorescamine.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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