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08951 Sigma-Aldrich

6-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥90.0% (HPLC)

Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

  • CAS Number 150206-15-8

  • Empirical Formula (Hill Notation) C29H19NO11

  • Molecular Weight 557.46

  •  MDL number MFCD00269772

  •  PubChem Substance ID 329748646

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescence, Fluorescent Labeling of Amines, Fluorescent Labels,
grade   for fluorescence
InChI Key   JGPOSNWWINVNFV-UHFFFAOYSA-N
assay   ≥90.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
storage temp.   −20°C

Description

Application

The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.

Other Notes

Reagent for continuously determining the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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