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100-05A Sigma-Aldrich

Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, adult

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   human neonatal foreskin or adult skin capillaries (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

General description

CADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.

Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).

Cell Line Origin

Skin

Application

Endothelial vessel function, cell signaling, angiogenesis

Components

Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the CADMEC/HMVEC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

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Protocols & Articles

Protocols

Protocol: Human Dermal Microvascular Endothelial Cells Culture

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