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10115 Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

lyophilized, powder, ~70 U/mg

Synonym: 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   powder
quality   lyophilized
mol wt   Mr ~97,000
optimum temperature   50 °C
storage temp.   2-8°C

Description

General description

Amyloglucosidase is a disaccharide enzyme belonging to the α-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger has been used in glycogen assay and in acetate buffer for starch hydrolysis.

Unit Definition

One unit corresponds to the amount of enzyme which liberates 1 μmole of glucose per minute at pH 4.8 and 60 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the molecular weight of Product 10115,Amyloglucosidase from Aspergillus niger?
Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
Does Product 10115,Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
Fluka does not add any extending additives such as glucose or sucrose. Only sodium acetate buffer salts are added. It is unknown to Fluka if the starting material contained any additives.
How can a solution of Product 10115,Amyloglucosidase from Aspergillus niger, be prepared?
A 1 mg/mL solution may be prepared in water, or in 10 mM sodium acetate buffer at approximately pH 4.8.
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Protocols & Articles

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Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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