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10771 Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

  •  NACRES NA.75

Purchase

Properties

Related Categories Cell Biology, Density Gradient Media and Cell Separation, Hematology and Histology, Histopaque
Quality Level   200
sterility   sterile-filtered
shelf life   Expiry date on the label.
application(s)   hematology: suitable
  histology: suitable
impurities   endotoxin, tested
density   1.077 g/mL
storage temp.   2-8°C

Description

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Histopaque-1077 has been used for-
• the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• purification of mononuclear cells through centrifugation
• isolation of human lymphocytes
• isolation of breast cancer circulating tumor cells (CTCs) from whole blood

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Is Histopaque®, Product 10771, the same as Ficoll® solution, Product F5415?
Ficoll-Hypaque is a mixture of Ficoll (polysucrose) and Hypaque (sodium diatrizoate). It does not specify a density. Product No. 10771 is a mixture of polysucrose and sodium diatrizoate at a density of 1.077 g/ml.
Should I use Histopaque® 10771 or 10831 for isolation of lymphocytes?
The different product numbers reflect the density of the solution. Product No. 10771 is to be used to isolate human mononuclear cells. Product No. 10831 is to be used for the isolation of mononuclear cells from mice, rats and other mammals.
What is the difference between Histopaque®Product No. 10771 and H8889?
Product 10771, Histopaque®-1077, is not cell culture tested and Product H8889, Histopaque®-1077 Hybri-Max, is cell culture tested.Both Productsare sterile, endotoxin tested and intended for Research Use Only.
What is the osmolarity of the Product 10771, Histopaque®-1077 solution?
The osmolarity is a property that is tested for each lot of material; the range of acceptable values is 280.0 - 300.0 mOs/kg.The lot specific value is given on the Certificate of Analysis.Certificates of Analysis can be viewed on our website by entering the lot number on our Advanced Search Page.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Histopaque® Troubleshooting Guide

The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by our Technical Support scientists based on observations and ex...
Mark Frei
BioFiles v6 n5, 14–16
Keywords: Adhesion, Anticoagulants, Cell culture, Centrifugation, Coagulation, Culture media, Sample preparations, Separation, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

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