10831 Sigma


sterile-filtered, density: 1.083 g/mL



Related Categories Cell Biology, Centrifugation Media, Core Bioreagents, Density Gradient Media and Cell Separation, Hematology and Histology,
Quality Level   PREMIUM
sterility   sterile-filtered
shelf life   Expiry date on the label.
impurities   endotoxin, tested
density   1.083 g/mL
storage temp.   2-8°C


General description

A solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. Facilitates the recovery of viable mononuclear cells from rats, mice, and other small mammals.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Are there limitations when attempting to use Product 10831, Histopaque®-1083 with the various Accuspin products?
Yes.There are some limitations.Small amounts of blood cannot be processed in the Accuspin tubes.At least 3 mls of whole peripheral blood is expected.Without sufficient red cell numbers, the mononuclear band fails to adequately clear the barrier or frit found within the Accuspin tubes.
I find mononuclear cells separate well from human blood overProduct 10831, Histopaque®-1083,but the cell recovery from rats and mice is lower.Why?
The most common cause for separation problems with Histopaque® 1083 is the blood source.Blood from rats and mice is harder to obtain than blood from a human.Most users experiencing problems are collecting blood with a syringe.It is relatively uncommon for users to call in about poor separation when using vacuum tubes.Humanely sacrifice the animal if possible and immediately collect the blood from the aorta or vena cava. Collecting blood from a severed head is never acceptable.Blood obtained from bleeding the tail is also generally unacceptable.Tissue fluids and other extraneous debris will often result in an activation of the coagulation cascase.Blood drawn into a vacuum tube is the best source of blood.See the product insert for Histopaque® 1083 for the amount of anticoagulant to add to blood collected with a syringe.
Can Product 10831, Histopaque®-1083,be used with human blood?
Cells can be recovered from human blood using Histopaque® 1083.However Sigma sells Histopaque® 1077 specifically for use with human blood.Histopaque® 1077 is intended for In Vitro Diagnostic Use.Histopaque® 1083 is intended for research use only with animal blood.
Is there any data for expected percent recovery with rat or mouse blood when using Product 10831, Histopaque®-1083?
No.Histopaque® 1083 is a research use product and no studies have been performed to determine percent recovery.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Milli-Q® Water Purification Solutions
Protocols & Articles


Histopaque® Troubleshooting Guide

The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by Sigma-Aldrich® Technical Services. These are based on observa...
Mark Frei
BioFiles v6 n5, 14–16
Keywords: Adhesion, Anticoagulants, Cell culture, Centrifugation, Coagulation, Culture media, Sample preparations, Separation, Sterilizations



Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?