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12023C Sigma-Aldrich

Newborn Calf Serum

New Zealand origin

Synonym: NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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Properties

Related Categories Cell Culture, FBS Premier, Fetal Bovine Serum (FBS) and Other Animal Sera, Fetal Bovine Serum (FBS) for cell culture, Newborn, Calf and Adult Bovine Sera More...
biological source   bovine (calf)
sterility   sterile-filtered
origin   New Zealand origin
application(s)   cell culture | mammalian: suitable
impurities   ≤35 EU/mL endotoxin
shipped in   dry ice
storage temp.   −20°C

Description

General description

Serum is mostly required as a supplement to cell culture media. It contains macromolecules, carrier proteins, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors.
Newborn Bovine Serum (NBS) is collected from calves that are less than 10 days old. Although NBS is not as nutritionally rich as Fetal Bovine Serum (FBS), it can serve as a cost-effective substitute for FBS in many applications.

Biochem/physiol Actions

Newborn Calf Serum has been used in FACS (fluorescence-activated cell sorting) study for resuspension and dilution of cells.

Linkage

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Analysis Note

• Endotoxin and hemoglobin tested
• Tested for the presence of bacteria, virus, mycoplasma, and hemoglobin content
• Triple filtered with 0.1 micron membrane under aseptic conditions

Endotoxin tested

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Serum for Cell and Tissue Culture: Frequently Asked Questions (FAQs)

Serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth and attachment factors. Serum also acts as a buffer to the cell cultu...
Keywords: Agriculture, Cell culture, Centrifugation, Culture media, Filtration, Nucleic acid denaturation

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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