• USA Home
  • 122-20 - Rat Tail Collagen Coating Solution (20 ML)

EMAIL THIS PAGE TO A FRIEND
122-20 Sigma-Aldrich

Rat Tail Collagen Coating Solution (20 ML)

  •  NACRES NA.75

Purchase

Properties

Related Categories Cell Culture, Primary Cell Culture, Reagents and Supplements More...
Quality Level   100
sterility   sterile; sterile-filtered
form   liquid
shelf life   6 mo.
packaging   pkg of 20 mL
application(s)   cell culture | mammalian: suitable
shipped in   wet ice
storage temp.   2-8°C
Gene Information   rat ... Col1a1(29393)

Description

General description

Enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. Commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.

Application

Coat tissue culture ware with Rat Tail Collagen Solution to culture surface ratio of 5 ug/cm2. For example: 125 ug (2.5 ml) for a T-25 flask. 10.5 ug (210 ul) for each well of a 24-well plate.

Coat tissue culture ware at 37oC for 2 hours. Carefully aspirate Collagen Coating Solution. Rinse the coated surface twice with PBS. Coated tissue culture ware may be used immediately or air-dried and stored at 4 oC for up to one week.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Introduction to Primary Cell Culture [VIDEO]

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cell culture, Drug discovery, Pharmaceutical

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?