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  • 14513 - 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrasodium salt

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14513 Sigma-Aldrich

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥95% (HPLC)

Synonym: 1,2-Bis (2-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetrasodium salt, BAPTA-Na4, Tetrasodium 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetate

  • CAS Number 126824-24-6

  • Empirical Formula (Hill Notation) C22H20N2Na4O10

  • Molecular Weight 564.36

  •  Beilstein/REAXYS Number 8818619

  •  MDL number MFCD00067691

  •  PubChem Substance ID 57647167

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Chelators and Ion Probes, Chelators and Ion Probes for Flow Cytometry, Chelators and Ion Probes for Microscopy, Detection,
Quality Level   200
assay   ≥95% (HPLC)
impurities   ≤12.5% water
solubility   H2O: 10 mg/mL, clear, faintly yellow
fluorescence   λex 254 nm; λem 363 nm in H2O
  λex 274 nm; λem 363 nm (with calcium)
storage temp.   −20°C
SMILES string   [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)c1ccccc1OCCOc2ccccc2N(CC([O-])=O)CC([O-])=O
InChI   1S/C22H24N2O10.4Na/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32;;;;/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32);;;;/q;4*+1/p-4
InChI key   ZWSMLJACYSGFKD-UHFFFAOYSA-J

Description

Application

BAPTA-Na4 is a fluorescent compound utilized to determine free Ca2+ by performing fluorescence emission scans at an excitation wavelength of 299 nm and measuring the emission wavelength at 360 nm .

suitable as Ca2+-indicator (fluorescence suppressed by Ca2+)

Other Notes

Chelator with a greater selectivity for calcium compared with magnesium (spectrophotometric monitoring):

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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