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  • 14533 - bisBenzimide H 33342 trihydrochloride

14533 Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

for fluorescence, ≥97.0% (HPLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

  • CAS Number 23491-52-3

  • Empirical Formula (Hill Notation) C27H28N6O · 3HCl · xH2O

  • Molecular Weight 561.93 (anhydrous basis)

  •  Beilstein/REAXYS Number 1234011

  •  EC Number 245-691-1

  •  MDL number MFCD00012678

  •  PubChem Substance ID 57647173



Related Categories Alphabetical, Biochemicals and Reagents, Detection, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains,
grade   for fluorescence
assay   ≥97.0% (HPLC)
fluorescence   λex 340 nm; λem 510 nm
  λex 355 nm; λem 465 nm in TE buffer; DNA
storage temp.   2-8°C



bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.


Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Product 14533, bisBenzimide H 33342 trihydrochloride?
It is soluble in water at 50 mg/mL.It will precipitate from phosphate buffer solutions
When Product 14533, bisBenzimide H 33342 trihydrochloride, is dissolved in water, what is the pH?
The pH of a 2% solution in water is approximately 1.9.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles


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