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14871 Sigma-Aldrich

BICINE

BioUltra, ≥99.5% (T)

Synonym: N,N-Bis(2-hydroxyethyl)glycine

  • CAS Number 150-25-4

  • Empirical Formula (Hill Notation) C6H13NO4

  • Molecular Weight 163.17

  •  Beilstein/REAXYS Number 1769362

  •  EC Number 205-755-1

  •  MDL number MFCD00004295

  •  PubChem Substance ID 57647225

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
InChI Key   FSVCELGFZIQNCK-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.1%
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   4.0-5.5 (25 °C, 1 M in H2O)
useful pH range   7.6 - 9.0
pKa (25 °C)   8.3
solubility   H2O: 1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤20 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.070
  λ: 280 nm Amax: 0.040

Description

Application

BICINE can be used to study biological buffers and zwitterionic compounds. N,N-Bis(2-hydroxyethyl)glycine has been used in a study that characterized refined crystal structures of human Ca2+/Zn2+-binding S100A3 protein. N,N-Bis(2-hydroxyethyl)glycine has also been used in a study to inform F1 antigen assembly.

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Related Content

BioUltra Biological Buffers

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Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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