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15205 Sigma-Aldrich

Blasticidine S hydrochloride

Synonym: Blasticidin S

  • CAS Number 3513-03-9

  • Empirical Formula (Hill Notation) C17H26N8O5 · HCl

  • Molecular Weight 458.90

  •  MDL number MFCD02091640

  •  PubChem Substance ID 57647341

Purchase

Properties

Related Categories A - K, Analytical Standards, Analytical/Chromatography, Antibacterial, Antibiotics,
InChI Key   YQXYQOXRCNEATG-ZAYJLJTISA-N
form   powder
color   white to off-white
solubility   acetic acid: water: soluble, clear, colorless
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   fungi
shipped in   dry ice
storage temp.   −20°C

Description

General description

Blasticidin S belongs to the aminoacylnucleoside class of antibiotics. It inhibits protein synthesis in bacteria and eukaryotes. It also has fungicidal properties and prevents rice blast disease.

Chemical structure: nucleoside

Application

Blasticidin S is used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or BSD. Blasticidiin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells . It is also used to study protein synthesis at the level of peptide bond formation.

Biochem/physiol Actions

Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
2

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is Product 15205, Blasticidine S hydrochloride, soluble in?
Blasticidine S hydrochloride should be soluble in water at a concentration of 5 - 10 mg/mL. It is also soluble in acetic acid (concentration not reported).
How should aqueous solutions of Product 15205, Blasticidine S hydrochloride, be stored?
Aqueous solutions of Blasticidine S hydrochloride should be stored in aliquots at -20 deg. C.Aqueous solutions at a pH < 7 are stable for 1 to 2 weeks at +4°C or for 6 to 8 weeks at -20°C.Avoid alkaline pH, and do not subject solutions to freeze-thaw cycles.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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