16016 Millipore


suitable for microbiology, NutriSelect® Basic

  •  NACRES NA.74



Related Categories A - M, All Liquid Media/Broths, Citrobacter, Escherichia coli & Coliforms, Fluorogenic Media,
form   powder
shelf life   limited shelf life, expiry date on the label
composition   brilliant green, 0.133 g/L
  lactose, 10 g/L
  4-methylumbelliferyl-β-D-glucuronide, 0.1 g/L
  ox-bile (dried), 20 g/L
  peptone, 10 g/L
  L-tryptophan, 1 g/L
mfr. no.   NutriSelect® Basic
application(s)   microbiology: suitable
final pH   7.2±0.2 (25 °C)
Featured Industry   Agriculture
Food and Beverages
storage temp.   room temp
suitability   selective and differential for Citrobacter spp.
  selective and differential for Escherichia coli
  selective and differential for Lactobacillus spp.
  selective and differential for Staphylococcus spp.
  selective and differential for coliforms



In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation.
The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.

Preparation Note

Dissolve 41 g in 1 litre distilled water and pour into tubesfitted with Durham tubes if necessary. Autoclave at 121°C.

Other Notes

Screening of bathing-water for coliforms according to EC directive

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Selective growth media for differentiation and detection of Escherichia coli and other coliforms

Normal constituents of the intestinal flora of animals, coliforms are rod-shaped, Gram-negative, non-spore forming facultative anaerobes. They ferment lactose with the production of acid and gas when...
Jvo Siegrist
AnalytiX Volume 8 Article 4
Keywords: Biochemistry, Clinical, Culture media, Electron capture detector, Fermentation, Immunology, Indicators, Microbiology, Molecular biology

Peer-Reviewed Papers


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