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16590 Sigma-Aldrich

Atto 520 maleimide

for fluorescence, ≥90% (coupling rate)

  • Empirical Formula (Hill Notation) C28H33ClN4O8

  • Molecular Weight 589.04

  •  PubChem Substance ID 329751273

  •  NACRES NA.32

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Properties

Related Categories Atto Labels, Biochemicals and Reagents, Fluorescence, Fluorescent Labeling of Sulfhydryls, Fluorescent Labels,
grade   for fluorescence
assay   ≥90% (coupling rate)
solubility   DMF: soluble
  DMSO: soluble
  acetonitrile: soluble
suitability   passes test for fluorescence
storage temp.   −20°C
SMILES string   [O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCN4C(=O)C=CC4=O)c2cc1C
InChI   1S/C28H32N4O4.ClHO4/c1-5-29-22-15-24-20(13-17(22)3)19(21-14-18(4)23(30-6-2)16-25(21)36-24)7-8-26(33)31-11-12-32-27(34)9-10-28(32)35;2-1(3,4)5/h9-10,13-16,29H,5-8,11-12H2,1-4H3,(H,31,33);(H,2,3,4,5)/b30-23-;
InChI key   DGEQWOHLXNEPEL-KZJPCHATSA-N

Description

Application

Atto 520 maleimide utilized for labeling sulfhydryl (thiol) groups of proteins, especially cysteine residues, to fluorescently study changes in conformation, binding, and kinetics .

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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