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16670 Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

≥95.0% (HPLC)

Synonym: BCIP® p-toluidine salt, X-phosphate p-toluidine salt

  • CAS Number 6578-06-9

  • Empirical Formula (Hill Notation) C8H6BrClNO4P · C7H9N

  • Molecular Weight 433.62

  •  EC Number 229-506-1

  •  MDL number MFCD00040636

  •  PubChem Substance ID 57647586

  •  NACRES NA.83

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Properties

Related Categories Alkaline Phosphatase, BCIP/NBT Substrate Systems, Biochemicals and Reagents, Detection Substrates, Enzyme Substrates,
assay   ≥95.0% (HPLC)
storage temp.   2-8°C
SMILES string   Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI   1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key   QEIFSLUFHRCVQL-UHFFFAOYSA-N

Description

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Packaging

1, 5 g in poly bottle

100 mg in poly bottle

Substrates

Histochemical substrate for alkaline phosphatase.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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