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  • 171M-9 - CD71 (MRQ-48) Mouse Monoclonal Antibody

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171M-9 Sigma-Aldrich

CD71 (MRQ-48) Mouse Monoclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CD0-CDC, Cell Marque Antibodies, Hematopathology,
conjugate   unconjugated
clone   MRQ-48, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (171M-94)
  vial of 0.5 mL concentrate (171M-95)
  bottle of 1.0 mL predilute (171M-97)
  vial of 1.0 mL concentrate (171M-96)
  bottle of 7.0 mL predilute (171M-98)
antibody form   culture supernatant
isotype   IgG1
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.01-1 μg/mL (predilute)
  10-30 μg/mL (concentrate)
control   bone marrow
visualization   cytoplasmic, membranous

Description

General description

The transferrin receptor (CD71) is most highly expressed on placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes, endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase. The maturation of erythrocytes results in loss of transferrin receptor expression, in concert with down-regulation of the machinery for hemoglobin synthesis. The high level of transferrin receptor within erythroid precursors makes it an excellent marker for evaluation of erythroid components within bone marrow. Anti-CD71 is a highly effective marker for highlighting erythroid precursors in bone marrow biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes do not express CD71, which facilitates bone marrow analyses.Anti-CD71 is useful in identifying erythroid precursors with very little interference from mature erythrocytes and also in the determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome, although further studies are needed for making a definitive diagnosis of MDS.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


IVD

IVD

IVD

RUO

Linkage

CD71 Positive Control Slides, Product No. 171S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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171S CD71 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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