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17490 Sigma-Aldrich

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

Synonym: 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

  • CAS Number 115-40-2

  • Empirical Formula (Hill Notation) C21H16Br2O5S

  • Molecular Weight 540.22

  •  Beilstein/REAXYS Number 359618

  •  EC Number 204-087-8

  •  MDL number MFCD00011681

  •  PubChem Substance ID 57647609

  •  NACRES NA.47

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, B, Base Ingredients for Media, Cell Biology,
Quality Level   200
grade   for microscopy (Hist., Vit.)
  indicator (pH 5.2-6.8)
form   powder
application(s)   hematology: suitable
  histology: suitable
  titration: suitable
pH range   5.2 - 6.8, yellow to purple
mp   240 °C (dec.) (lit.)
solubility   hot ethanol: 0.1 g/10 mL
εmax   ≥400 at 414-424 nm in methanol
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
InChI   1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
InChI key   ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Application

Spectrophotometric determination of serum-albumin

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Peer-Reviewed Papers
15

References

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