EMAIL THIS PAGE TO A FRIEND
17783 Sigma

Calcein-AM

BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

Synonym: Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, Calcein acetoxymethyl ester

Purchase

Properties

Related Categories Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Biochemicals and Reagents, Calcium Indicators, Cell Culture, Cell Viability,
InChI Key   XKFSBWQWNMZWFA-UHFFFAOYSA-N
product line   BioReagent
assay   ≥96.0% (HPLC)
fluorescence   λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Calcein-AM has been used in flow cytometry.

General description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

What is the solubility of Calcein-AM, Product 17783?
Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).
 What is the appearance of Calcein-AM, Product No. 17783?
Product No. 17783 can be a film, oil, paste or solid.
What are the spectral properties of Calcein-AM, Product 17783?
The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).
How is Calcein-AM, Product 17783, typically used?
Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Do the Scienceware® brand polypropylene products contain any leachable material?  
Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.
Show more questions
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Detect, Visualize and Quantify Single Post-Translational Modifications

Duolink® In Situ products enable detection, visualization, and quantification of protein events in tissue and cell samples prepared for microscopy. The in situ PLA® technology, on which these product...
Caleb Hopkins, Product Manager, Sigma® Life Science
Biofiles Vol. 8, No. 11
Keywords: Acetylations, Amplification, Apoptosis, Cancer, Cellular processes, Clinical, Diagnostic, Diseases, Enzyme activity, Gene expression, Glycosylations, Immunohistochemistry, Immunoprecipitation, Microscopy, Nucleic acid hybridization, Peptide synthesis, Phosphorylations, Post translational modifications, Transduction, Ubiquitinations, Western blot

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?