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18507 Sigma-Aldrich

Atto 565 maleimide

BioReagent, suitable for fluorescence

  • Empirical Formula (Hill Notation) C37H37ClN4O10

  • Molecular Weight 733.16

  •  MDL number MFCD04040675

  •  NACRES NA.32

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Properties

Related Categories Atto Labels, Biochemicals and Reagents, Fluorescence, Fluorescent Labeling of Sulfhydryls, Fluorescent Labels,
Quality Level   200
product line   BioReagent
fluorescence   λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
suitability   corresponds for coupling to thiols
  suitable for fluorescence
storage temp.   −20°C

Description

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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