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21878 Sigma-Aldrich

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym: 5(6)-FAM SE, FLUOS

  • CAS Number 117548-22-8

  • Empirical Formula (Hill Notation) C25H15NO9

  • Molecular Weight 473.39

  •  MDL number MFCD04119713

  •  PubChem Substance ID 57647947

  •  NACRES NA.32

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Description

Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.

Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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