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21884 Sigma-Aldrich

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

BioReagent, suitable for fluorescence, ≥85% (HPCE)

  • CAS Number 127770-45-0

  • Empirical Formula (Hill Notation) C25H14Cl2O9

  • Molecular Weight 529.28

  •  Beilstein/REAXYS Number 7505106

  •  MDL number MFCD08459585

  •  PubChem Substance ID 57647949

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Detection, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Flow Cytometry,
product line   BioReagent
assay   ≥85% (HPCE)
mp   210 °C (dec.) (lit.)
solubility   DMF: soluble
  DMSO: soluble
  methanol: soluble
fluorescence   λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)
suitability   suitable for fluorescence
storage temp.   −20°C
SMILES string   CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)c%10ccc(cc9%10)C(O)=O)c7cc6Cl
InChI   1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
InChI key   LZMPJBOMBCYYMP-UHFFFAOYSA-N

Description

Application

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDCF) is used as a fluorescent substrate/probe to study the distribution and mechanism of action of multidrug resistance-associated protein 2 (Mrp2).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

Excmax: 504 nm (Lit.)

Other Notes

Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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