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21888 Sigma

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: CFSE

Purchase

Description

Application

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?
We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.
What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?
Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.
Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?
Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.
Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?
No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Common Cell Culture Problems: Cell Clumping

Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in the culture medium, which...
Keywords: Adhesion, Apoptosis, Cell culture, Cell disruption, Culture media, Environmental, Flow cytometry

Common Cell Culture Problems: Cell Death

There is perhaps nothing more frustrating to a cell culturist than removing a dish from the incubator only to find that it’s full of dead cells. Getting to the bottom of the problem can be very chall...
Keywords: Antibiotics, Apoptosis, Autophagy, Buffers, Cell culture, Cell disruption, Cryopreservation, Culture media, Environmental, Enzyme activity, Filtration, Metabolism, Peptide synthesis, Reductions

Common Cell Culture Problems: Cell Line Cross-Contamination and Misidentification

A few years after scientists working at Johns Hopkins established the first human cell line using cervical epithelial cells (HeLa) from cancer patient Henrietta Lacks in the early 1950s, it was obser...
Keywords: Cancer, Cell culture, Cryopreservation, Forensic, Genetic, Phase transitions, Polymerase chain reaction

Common Cell Culture Problems: Contamination

Overview Types of Contamination Common Causes and Prevention of Contaminants    Microbial (bacteria, fungi, yeast)    Mycoplasma    Viral Contamination    Chemical Contamination General Tips and Tech...
Keywords: Antibiotics, Antifungals, Apoptosis, Buffers, Cell attachment, Cell culture, Culture media, Detergents, Eliminations, Environmental, Filtration, Fluorescent microscopy, Gene expression, Indicators, Metabolism, Microscopy, Polymerase chain reaction, Sterilizations

Common Cell Culture Problems: Poor Attachment of Adherent Cells

Optimal cell attachment in vitro requires the interaction of healthy cells with a wide range of cell-derived attachment molecules present in media, serum, and supplements. Cultureware surfaces are fr...
Keywords: Buffers, Cell attachment, Cell culture, Culture media, Decarboxylations, Environmental, Growth factors, Lipid peroxidation, Peroxidations, Precipitation, Reductions, Respiration

Common Cell Culture Problems: Precipitates

When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, a...
Keywords: Antibiotics, Cell culture, Culture media, Desiccation, Evaporation, Filtration, Microscopy, Nucleic acid denaturation, Precipitation, Purification

Protocols

Common Cell Culture Problems: Poor Cell Growth

Ensuring adequate cell growth is a critical part of collecting accurate data with cell cultures. Cells can be cultured in suspension, or as a monolayer that attaches to cultureware, such as a flask, ...
Keywords: Antibiotics, Apoptosis, Buffers, Cell attachment, Cell culture, Condensations, Cryopreservation, Culture media, Evaporation, Filtration, Microscopy, Phase transitions, Reductions

Peer-Reviewed Papers
15

References

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