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  • 221M-1 - Annexin A1 (MRQ-3) Mouse Monoclonal Antibody

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221M-1 Sigma-Aldrich

Annexin A1 (MRQ-3) Mouse Monoclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Cell Marque Antibodies, Hematopathology,
conjugate   unconjugated
clone   MRQ-3, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (221M-14)
  vial of 0.5 mL concentrate (221M-15)
  bottle of 1.0 mL predilute (221M-17)
  vial of 1.0 mL concentrate (221M-16)
  bottle of 7.0 mL predilute (221M-18)
antibody form   culture supernatant
isotype   IgG1
Quality Level   100
  500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.01-0.1 μg/mL (predilute)
  1-10 μg/mL (concentrate)
control   hairy cell leukemia
visualization   cytoplasmic, membranous

Description

General description

ANXA1 is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumor cells in 97% of samples from patients with hairy cell leukemia. By contrast, B-cell lymphomas other than hairy cell leukemia, including typical splenic lymphoma with villous lymphocytes and patients with variant hairy cell leukemia—as defined by current morphologic, phenotypic, and clinical criteria—are ANXA1-negative. In a study by Falini et al. ANXA1 immunodetection was 100% sensitive and specific for hairy cell leukemia. Normal B cells from different lympho-hemopoietic tissues were ANXA1-negative. In this study the expression of ANXA1 in myeloid cells, macrophages, or T-cell subset served as positive control. These findings validated the results of gene expression profiling in hairy cell leukemia at the protein level by showing that ANXA1 is consistently expressed in this type of leukemic disease, but not in other B-cell lymphomas. Of note is that negativity for ANXA1 was also present in patients with splenic lymphoma with villous lymphocytes, variant hairy cell leukemia, prolymphocytic leukemia, marginal zone and lymphoplasmacytoid lymphomas. Thus, ANXA1 is a molecule specific to hairy cell leukemia that can be used to differentiate this disease from other B-cell lymphomas. Wang et al. showed that high ANXA1 expression is frequent in esophageal and esophagogastric junction adenocarcinomas, is associated with more advanced pathologic T stage and the presence of distant metastasis, and is an independent prognostic factor for patient survival.
Associated products: TRAcP, T-bet.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


IVD

IVD

IVD

RUO

Linkage

Annexin A1 Positive Control Slides, Product No. 221S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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Description

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221S Annexin A1 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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