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27815 Sigma-Aldrich

Brilliant Blue G

250, for microscopy

Synonym: Acid blue 90, Coomassie Brilliant Blue G

  • CAS Number 6104-58-1

  • Empirical Formula (Hill Notation) C47H48N3NaO7S2

  • Molecular Weight 854.02

  •  Colour Index Number 42655

  •  Beilstein/REAXYS Number 5230822

  •  EC Number 228-058-4

  •  MDL number MFCD00078482

  •  PubChem Substance ID 329752898

  •  NACRES NA.26

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Properties

Related Categories Molecular Biology, Protein Sequencing, Protein Structural Analysis, Proteomics, Reagents for Protein Sequencing More...
grade   for microscopy
SMILES string   [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
InChI   1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
InChI key   RWVGQQGBQSJDQV-UHFFFAOYSA-M

Description

General description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

Analysis Note

λmax. ∼610 nm; E(1%,1 cm) ∼500-700 (H2O)

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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