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30574 Sigma-Aldrich

Atto 488-Biotin

BioReagent, suitable for fluorescence, ~75% (HPCE)

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Properties

Related Categories Biochemicals and Reagents, Biotin Conjugates, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains
Quality Level   100
product line   BioReagent
assay   ~75% (HPCE)
fluorescence   λex 501 nm; λem 523 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 488 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Superior Fluorescent Imaging Using Atto Dyes

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Bacterial conjugations, Building blocks, Degradations, Enzyme-linked immunosorbent assay, Extinction coefficient, Flow cytometry, Fluorescent microscopy, Immunohistochemistry, In Situ hybridization, Indirect ELISA, Infrared spectroscopy, Microarray Analysis, Microscopy, Nucleic acid hybridization, PAGE, Separation, Western blot

Peer-Reviewed Papers
15

References

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