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308-05A Sigma-Aldrich

Human Internal Thoracic Artery Endothelial Cells: HITAEC, adult

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   human intrathoratic artery (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cardiovascular diseases, diabetes
shipped in   dry ice
storage temp.   −196°C

Description

General description

HITAEC has been used to investigate oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4 (Miles, 2014). To identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis, HITAEC and HCAEC (derived from the athero-prone human coronary arteries) were subjected to microarray analysis (Archaki, 2012). Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.

Cell Line Origin

Artery

Application

coronary artery bypass grafting, myocardial revascularization, endothelial cell motility, transendothelial migration, gene expression, contractile responses, receptor stimulation, cell relaxation, supply of oxygenated blood to chest wall and breasts,

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HITAEC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles

Protocols

Human Internal Thoracic Artery Endothelial Cells (HITAEC) Culture Protocol

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