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  • 308M-1 - Oct-2 (MRQ-2) Mouse Monoclonal Antibody

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308M-1 Sigma-Aldrich

Oct-2 (MRQ-2) Mouse Monoclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, Hematopathology, O,
conjugate   unconjugated
clone   MRQ-2, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (308M-14)
  vial of 0.5 mL concentrate (308M-15)
  bottle of 1.0 mL predilute (308M-17)
  vial of 1.0 mL concentrate (308M-16)
  bottle of 7.0 mL predilute (308M-18)
antibody form   diluted ascites fluid
isotype   IgG1κ
Quality Level   100
  500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.25-1.5 μg/mL (predilute)
  10-50 μg/mL (concentrate)
control   tonsil
visualization   nuclear

Description

General description

Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. These are involved in proliferation and differentiation and despite the scarce evidence for Oct-2 expression in T cells, it has been shown that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and alternatitive splicing, although it seems that the level of its expression can be used as a marker of B-cell lineage and differentiation. The following show high levels of Oct-2 expression: germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, classic Hodgkin lymphoma.

Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines display high levels of expression. Analysis of Oct-2 expression in primary Hodgkin’s lymphoma (HLs) and cell lines derived from it showed that the tumor cells analyzed had high levels of expression and activity, suggesting a common B-cell origin for all types of HLs.

Associated products: bcl-6, CD20, CD79a, PAX-5, BOB.1

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


IVD

IVD

IVD

RUO

Linkage

Oct-2 Positive Control Slides, Product No. 308S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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Description

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308S Oct-2 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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